Direct Marketing Assignment Example | Topics and Well Written Essays - 1500 words

Direct Marketing - Assignment Example 1) Cost – As the cost of per target promotion in direct marketing tends to be substantially higher than that in case of mass marketing, marketers must send their message to a limited audience only, as sending the message to general audience at large with very few of them likely to respond positively or getting converted may increase the cost manifold and thus cause a lot of marketing dollar wastage. 2) Customer irritation – As direct marketing communication tends to be longer and more involving i.e. seek active participation of the customer, than traditional mass marketing communication, thus unnecessary and unsolicited message may lead to customer frustration, resultantly eroding brand equity. Thus in direct marketing it is important to come up with a list of the customers who would be most interested in the product offering and thus will be most likely to respond positively rather than non-responding or responding negatively by avoiding further communication. â€Å"Data Mining is the process of discovering interesting knowledge from large amounts of data stored either in databases, data warehouses, or other information repositories. Simply stated, data mining refers to extracting or "mining" knowledge from large amounts of data.† (Han and Kamber, 2006). According to Fayyad et al (1996), Data mining is an important process of discovering novel, implicit, useful and comprehensive knowledge from large amount of data. For Direct marketing it translates in to a description of likely buyers or responders and thus Data mining helps in securing better results, for a direct marketing campaign than a traditional mass marketing campaign For example - To develop marketing and service –delivery strategy, Harrah’s entertainment Inc., the gambling and hotel chain uses Data mining to target customers on individual basis for direct marketing. Using its sophisticated

Assingment 3 Assignment Example | Topics and Well Written Essays - 500 words

Assingment 3 - Assignment Example A favorable relationship allows the manager to have a task structure that is highly formed and he is able to punish or reward the employees. An unfavorable relationship is usually associated with a poor task structure and the leader has limited authority. 3) Positioning power-, this variable usually measures the amount of power and authority that a manager perceives the organization has granted him for the purpose of rewarding, directing, and punishing of subordinates. Positioning of managers usually depends on the increment of the decision making power of employees. Path goal is a theory that was developed in order to describe the way leaders are able to support and encourage their followers in the achievement of goals that have been set by making the path of success easy and clear (House, 1996). The leader in this case is supposed to, This type of leadership involves the consideration of the follower’s needs, showing concern for their wellbeing and the creation of a working environment that is friendly. This will include the increment of the self-esteem for the followers and enhancing job roles to make work more interesting. This usually works best in a scenario whereby the work is hazardous, boring, or stressful. Directive leadership is the kind of leadership whereby the leader tells the followers what is supposed to be done and helps in guiding them along the way. This will include schedules for specific work and specific time. This involves the consultation of followers and asking for ideas and considering them in the process of decision-making. This approach usually works best in a situation whereby the followers are experts in a certain field. This involves the setting up of goals that are challenging for self-improvement and at work. In this case, high standards are usually expected and demonstrated. The leader should show faith in capabilities of the follower. This approach is best in a complex job

Organizational Culture Essay Example for Free

Organizational Culture Essay Organizational culture is the sum total of the organization’s past and current assumptions, experiences, philosophy, and values that hold it together and are expressed in its self-image, inner workings, interactions with the outside world, and future expectations. It is based on shared attitudes, beliefs, customs, express or suggest contracts, written and unwritten rules that the organization develops over time and that have worked well enough to be considered valid. Culture is a very powerful force at the workplace, which is consciously and deliberately cultivated and is passed on to the incoming employees. It reflects the true nature and personality of an organization. Basically, organizational culture is the personality of the organization. Culture is comprised of the assumptions, values, norms and artifacts of organization members and their behaviors. Members of an organization will sense the particular culture of an organization soon. Culture is one of those terms that are difficult to express distinctly, but everyone knows it when he or she sense it. For example, the culture of a large profit corporation is quite different than that of a hospital which is quite different from that of a university. You can tell the culture of an organization by looking at the arrangement of furniture, from employees behavior, what members wear, and many more. Similar to what you can use to get a feeling about someones personality. Corporate culture can be looked at as a system. Inputs include feedback from, for e.g., society, professions, laws, stories, heroes, values on competition or service, and many more. The process is based on our assumptions, values and norms, e.g., our values on money, time, facilities, space and people. Outputs or effects of our culture are, e.g., organizational behaviors, technologies, strategies, image, products, services, appearance, and many more. The concept of culture is particularly important when attempting to manage organization-wide change. Practitioners are coming to realize that, despite the best-laid plans, organizational change includes not only changing structures and processes, but also the corporate culture. Theres been a great deal of literature generated over the past decade about the concept of organizational culture particularly in regard to learning how to change organizational culture. Organizational change efforts are said to fail the majority of the time. Usually, this failure is credited to lack of understanding about the strong role of culture and the role it plays in organizations. Thats one of the reasons that many strategic planners now place as much emphasis on identifying strategic values as they do mission and vision. To a lesser degree, an organizational structure can get in the way of, or support, the overall success of your projects. This is a lesser problem because, to a certain extent, you can change your organizational structure. In fact, you can change the organization chart frequently, and some companies do just that. Culture, on the other hand, is not easily changed. It can take years for a large organization to develop a culture of excellence. Some organizational structures can definitely impair your ability to deliver projects. First are those organizations whose project teams are doing to support work. If your project organization does support as well, it usually means that support issues will pop up and take the focus away from the project. A lot of multitasking and thrashing takes place as you move from support work to project work to support work. It’s usually very difficult to prepare good estimates and meet your scheduling commitments. You may be forced into this structure if your staff is small. For example, a company for instance, has 15 people who worked on support, projects, and enhancements. However, they didn’t have enough people to specialize in either support or project work. This made it difficult to meet all of the project commitments. Instead, employees had to do a good job of managing expectations. Organizational structure may also prevent the ability to share resources. For instance, if your project team needs a resource with a specific expertise, you may not be able to easily share that person with another functional area. Some of this is also related to your culture. Ask yourself whether a different organizational structure would help. If it would, you may have an organization problem. If it wouldnt help, your culture is probably not supportive of resource sharing. Creating positive culture in an organization is a competitive advantage. Organizations with a positive culture are proven to be more productive. Many organizations are implementing participative management. More recently, the concept of empowerment has added to participative management by encouraging employees to internalize their organizations culture and make independent decisions. Empowerment can be an integral element of organizational culture change. Good leaders and good followers are necessary for empowerment to be influential in developing an organization with a strong culture. Experts suggest managers with empowered people on their staff are more effective. They achieve more of their objectives and they achieve them more easily. Empowerment embodies the concepts of internal motivation, internal justification for decision making, shared responsibilities, and integration for problem solving. As employees mature in an organization, they gain more knowledge, internalize justification for the actions they take, and become more intrinsically motivated. Besides this internalization process, employees tend to take a more active role in intervening in the actions of newer employees and offering feedback regarding culture-consistent behaviors. Managers should recognize this model in their organizations and manage their employees accordingly. Reference Jeffrey Kerr. (1987). Managing corporate culture through reward systems. Academy of management Executive, 1(2), 99-108. Daft, R.L. (2008). Management. Canada: Nelson Education. Heithfield, Initials. (2006). culture enviorment. Retrieved from http://humanresources.about.com/od/organizationalculture Freeman, Initials. (2008). Environment ethics and business . 5-10. Schachter, Initials. (2005). The importance of understanding organizational culture. Retrieved from http://findarticles.com/p/articles/mi_m0FWE/is_6_9/ai_n1

Picornavirus Life Cycle and Processing of Proteins

Picornavirus Life Cycle and Processing of Proteins Introduction Picornaviruses are small positive strand RNA viruses with single stranded genomic RNA capable of causing various diseases in humans (Porter, 1993). The picornavirus RNA genome has 3 poly(A) tail and a virus encoded particle Vpg at the 5 end. Unlike other RNA genome viruses, picornavirus doesnt contain a 7- methyl guanosine cap at the 5end intead they contain VPg a viral protein linked at the 5 end of the genome (Bedard and Semler, 2004). IRES (Internal ribosome entry site) is a clover leaf secondary structure at the 5 non-coding region and in the polypeptide there is a 3 noncoding region which contains the poly(A) tract required for the synthesis of minus RNA strand for RNA replication and translation. The minus strand RNa increases the efficiency of RNA replication and translation. The structural and non structural proteins are found at the polyprotein and the polyprotein is divided into three regions such as P1, P2, P3 where P1 contains the structural proteins (VP1, VP2, VP3 and VP 4) required for capsid formation and the non structural proteins P2 and P3 are required for replication and membrane rearrangement. The P2 and P3 region contains proteinase 3C and viral RNA dependent polymerase 3D (Stanway, 1990). There are 11 mature polypeptides with three main cleavage intermediates. UTR- Untranslated region; IRES- Internal ribosome entry site; VPg- Viral protein genome-linked (Lin et al, 2009). Processing of proteins The processing of proteins starts from the primary cleavage occurring between P1 and P2, mediated by viral protease 2A containing the cysteine nucleophile and it cleaves P1 and P2 leaving the viral polyprotein at cis. The P2 and the P3 precursors are separated from P1 region. For aphthovirus self cleavage takes place at the P1 domain region of the polyprotein by L proteinase at the cis whereas the L proteins in cardiovirus possess no proteolytic activity and hence in aphthovirus the cleavage of P1 is initiated by proteinase 3C. 2A proteinase also cleaves the cellular factors in entero and rhinovirus where eIF4G, a cap binding complex is cleaved and due to this even the there is a shut off in host translation. The L proteinase also cleaves eIF4G in aphtho virus during primary cleavage (Ryan and Flint,1997). There are four structural proteins in Picornavirus capsids such as VP1, VP2, VP3 and VP4 where the VP4 protein is inside of the capsid. The VP4 proteins are formed by the cleavage of VP0 precursor, late during the assembly and are modified after translation by the covalent attachment of myristic acid at the amino terminus (Cann, 1997). Picornaviruses consist of three types of proteinase L, 2A and 3C. the aphtho or F M D V polyproteins are processed by more than one proteinase. The L proteinase are available in two forms Lb pro and Lab pro. L pro possess the same function as 2A proteinase of entero and rhinovirus which cleave the host cell protein eIF4G but the major difference between L pro and 2A is that the L pro cleave in between Gly479 and Arg470 residues whereas 2A cleave in between Arg486 and Gly487. Lb pro plays a major role in substrate binding and also in shut off host cell translation like that of 2A proteinase (Ryan and Flint,1997). 2A proteinase cleaves at its own N terminus and the primary cleavage is carried at the P1 capsid protein precursor. The nature of 2A proteinase is unclear and the sequence similarities led to the understanding that 2A pro catalytic triad composed of His20, Asp38 and also an active site nucleophile of cysteine. 2A pro can be inhibited by active thiol proteinases such as iodoacetamide and N-ethylmaleimide. 2A proteinase also inhibits the host cell protein synthesis which mediated the cleavage of eIF-4G, a 220K Da polypeptide but it was later understood that the 2A pro just initiated as an activating factor for another proteolytic activity to cleave eIF-4G. Generally 2A pro shuts off the host cell translation because eIF-4G deals with cap dependent mode of translation in host cell. In poliovirus, 2A acts a trans activator of translation at IRES when host cell is not imhibited. When 2A pro was mutated it led to loss of cleavage activity in trans but not in cis, and no replication of vira l RNA was seen hence this confirms that 2A pro is required for viral RNA replication. The aphtho and cardiovirus 2A proteinase show no sequence similarity to entero/rhino virus although 2A protease are similar in size. The primary cleavage of hepatovirus and echovirus does not take place by the cleavage of 2A proteinase and the 2A protein in hepatovirus and echovirus showed no proteolytic activity (Ryan and Flint,1997). 2B and its precursor 2BC is a viral protein consisting of two hydrophobic regions with ÃŽ ± amphipathic a-helix domains leading to the alteration of membranes in the infected cells. The virus induced vesicles are formed when 2B and its precursor 2BC enter into the host membrane of Golgi and ER complex by altering the permeability of plasma membrane to form virporin complex. Once the 2B and the precursor 2BC enters the host there is an imbalance in Ca2+ homeostatis mechanism and blocks protein transport from ER to Golgi and also initiates anti-apoptosis property. The 2B protein also blocks the activation of IRF-3 in Hepatitis A virus through which the cellular IFN-ÃŽ ² gene transcription is inhibited so that there is no harm to Hepatitis A virus in the host. (Lin et al, 2009). The vi ral RNA was anchored for the spatial arrangement required for replication by the 2C protein. The mutagenesis experiments confirmed that the 2C proteins are involved in strand separation of viral RNA while replication (Porter, 1993) Secondary cleavage: The secondary cleavage is carried out within the viral proteins and hence it is mediated by 3C proteinase which plays a very important role in protein processing and RNA replication. The replication proteins are generated within the P2 and P3 precursor proteins when 3C self cleaves at the P3 region of the polyprotein. 3C proteinase or the 3CD precursors cleaves the poly(A) binding protein to inhibit viral translation during late poliovirus infection. 3C also cleaves the host cell protein required for transcription. The key processing step for the viral protein processing cascade is initiated by 3C proteinase. The 3CD proteins also play a major role in carring out important functions during RNA replication (Bedard and Semler, 2004). The major function of 3C proteinase in secondary cleavages is that it process the capsid and the replicative protein precursors. The processing of capsid in poliovirus is done by 3CD proteinase and not 3C proteinase. The 3C proteins are also used to cleave various number of host cell proteins such as histone H3, transcription factor IIIC, TATA binding protein and microtubule-associated protein 4. The 3CD proteinase depends on the host cell protein EF-1 ÃŽ ± and the host cell factor is replaced by 3CD forming 3AB:3CD proteinase complex to bind at the 3 end of the poliovirus genome (Ryan and Flint, 1997). The 3A protein inhibits the cellular protein function and also presents the membrane proteins during viral infection. When the poliovirus 3A protein was mutated, the uridlylation of VPg was affected and also inhibited the viral RNAs plus strand initiation (Porter, 1993). 2B is a viral protein which is required for virion release by altering the cell membrane increasing permeability which is required for poliovirus RNA replication. 2C proteins and its precursor 2BC is necessary for the re-arrangement of intracellular membranes and also for the viral induced cytoplasmic vesicles. 2C binds with the minus strand of poliovirus RNA at the 3 non coding region and hence it plays a role in positive RNA viral strand synthesis and also in minus strand RNA synthesis. 3C and 3D protein helps in immune response interference and also in viral RNA replication. 3A protein is very much important because these proteins help the picornaviruses to escape from MHC-I ( Major histo compatability) expression and intracellular membrane transport by inhibiting both the MHC-I and intracellular membrane transport of the host cell. 3B protein also called as VPg is linked to the 5 end of both the positive and negative strand RNAs. 3AB, 3C, 3CD and 3D are required during the proces s of assembly in replication and also initiates viral RNA polymerase 3D and self cleavage of 3CD. 3C and 3D are involved in binding viral RNA, protein processing and RNA replication. The cloverleaf structure of poliovirus virus and coxsackievirus consist of stem loops in which the viral polymerase precursor, 3CD binds to the stem loop I. 3CD also binds with the host cell protein poly r(C) binding protein 2 (PCBP2) to help only in RNA replication. 3D is responsible for VPg uridylylation and RNA chain elongation while synthesising viral RNA because it contains RNA dependent polymerases and even tends for error prone and mis-incorporation of 1-2 nucleotides per replication (Bedard and Semler, 2004). VP1, VP2 and VP3 are the three larger capsid proteins folded into eight stranded antiparallel ÃŽ ² barrels and a small fourth protein called VP4 is located inside the capsid. The 3C protease cleaves VP3, VP1 and VP0 at the P1 region. The amino termini of VP0, VP3, VP1 initiates the assembly of the virion particle. VP0 peptides are cleaved into VP2 and VP4 at the final stage of processing and assembly. VP4 and VP2 are adjacent to each other at their ends when cleaved. The infection of the host is initiated when the capsid proteins bind to the receptor on the host membrane. ICAM-1 (intercellular adhesion molecule 1) is the receptor molecule for the major rhinovirus which binds the cell to adjacent substrates. Poliovirus receptor molecule is an integral membrane protein consisting of one variable and two constant domains and this receptor molecule attaches to the host cell to initiate replication process. The general receptors used by the various picornaviruses are poliovirus receptor f or poliovirus attachment, ICAM-1 receptor for major rhinovirus, LDL-R for minor group rhinovirus, CD55 or DAF receptor for some echoviruses and group B coxsackie B1-B6 receptors. A deep cleft known as canyon is formed by flanking the monomers, VP1, VP2 and VP3 which helps the virus to escape the immune response by the host cell. The interaction of capsid proteins with the intracellular host factors affects the induction of apoptosis (Lin et al, 2009). Host cell shut off mechanism The mRNA of picornavirus is uncapped and hence the translation takes place by directly introducing ribosomes at internal ribosome entry sites (IRES). The cleavage of eukaryotic translation initiation factor, eIF4G by 2A protease inhibits the cap dependent mRNA translation of the host cell. When poliovirus is introduced into the host it inhibits the host cell translation leading to apoptotic cell death. The eIFGII is more resistant to the infection of poliovirus than eIFGI. The death inducing proteins encoded by cellular mRNA were translated by cap independent translation leading to apoptotic death. The cleavage of eIF4GI by caspase 3 also induces apoptotic cell death but differs from poliovirus 2A protease process. Severe inhibition of translation leading to apoptosis is seen by both the caspase 3 activity and 2A protease. IRES elements in mRNAs encode proteins which regulate apoptotis. Even poly (A) protein and dystropin protein are also cleaved by 2A protease.. Hence, the cleavage of poly(A) binding protein by 2A protease cause apoptotis and the cleavage of dystrophin protein can induce apoptotic process due to cytoskeleton disruption (Goldstaub et al, 1999). The enterovirus 2B protein suppresses apoptotic pathway of the host cell by controlling intracellular Ca2+ homeostatis. The apoptotic responses are initiated by the 3C and 2A proteinase to inhibit cellular transcription and cap dependent translation. When the 2B proteins are suppressed by caspase-3 activation it leads to apoptotis (Campanella et al, 2004). The cellular mRNAs encode for the death inducing proteins which are translated by cap independent translation. When eIF4GI and eIF4GII are cleaved by 2A protease it leads to apoptotic death because it inhibits cap dependent translation. The 2A proteins also cleave the poly(A) binding protein and the dystropin protein which leads to apoptotis through a translational mechanism (Goldstaub et al, 2000). The eIFE is the component of cap binding complex of cap structure at 5 end of mRNA. The 40 S ribosomal subunit checks at the 5 non coding region until it finds the initiating codon or the the authentic start codon AUG and then sends a signal to the 60S to form a complex. For translation to occur in mRNA the 5 non coding region with the cap end should bind with the eIFE and once it binds to the cap the 40 S ribosomal subunit scans for the authentic initiation or start codon AUG and once it finds the AUG codon it gives a signal to 60 S ribosomal subunit to form a complex with 40 S and initiate the translation along with initiation factors (Bedard and Semler, 2004). In picornavirus the 5 end of mRNA in the non coding region is not capped and hence to initiate translation, the cap independent mechanism is required. Hence in picornavirus, the 40 S ribosomal subunit scans for ribonucleo protein complex at 5 non coding region and initiation take place to recognise authentic start codon. The eIF4G is cleaved by viral proteinase 3C and 2A which shuts off the host cell translation (cap dependent) and also cleaves poly(A) binding protein (PABP)and hence it inhibits the host cell translation. When the host cell is infected by Foot and mouth disease virus (FMDV), the eIF4G subunit which is responsible for the cap dependent translation of the host is cleaved by the L protein and for the Encephalomyocarditis virus (EMCV), the cap dependent translation of host is blocked by a repressor protein 4E-BP1 which binds at the 5 cap region and hence the repressor protein 4E-BP1 blocks the binding of eIF-4E which is the cap binding subunit and hence the host translation is shut off. The Hepatitis A virus (HAV) does not shut off the host translation because it requires eIF4G for its translation (Bedard and Semler, 2004). All picornavirus consist of internal ribosome entry site (IRES) and are mapped to the 5 non coding region. There are four types of IRES seen in picornaviruses based on the RNA secondary structures. The enterovirus and rhinovirus comes under the Type I IRES. Aphthovirus and cardiovirus comes under the Type II IRES and the Hepatitis virus comes under the Type III IRES. The porcine reschovirus comes under the Type IV elements. The IRES mediated translation is initiated by two factors Canonical initiation factors and IRES trans-activating factors. Canonical initiation factors to initiate IRES mediated translation: The IRES elements of poliovirus and EMCV are similar and require the initiation factors to be primed at the 40S ribosomal subunit. The IRES mediated translation is initiated by certain canonical factors such as eIF4G and eIF4B to bind to the viral RNA and also certain other subunits such as eIf3 and eIF2 to pre-bind to the 40 S ribosomal subunits. The IRES translation of poliovirus and EMCV is promoted by the poly(A) binding protein (Lin et al, 2009). Noncanonical initiation factors to initiate IRES mediated translation: The polypyrimidine tract-binding protein (PTB) is a 57 KDa mRNA splicing factor which increases and promotes the activity of IRES in poliovirus. The molecular switching from translation to the replication of poliovirus is done by the proteolytic cleavage of PTB. The PTB also functions as RNA chaperon by stabilizing the type II IRES of FMDV and EMCV. Lupus autoantigen (La) is a 52 KDa of nuclear protein which binds to certain distinct parts of HAV IRES and with small interfering RNA and hence the HAV IRES translation and replication is suppressed. Poly(rC) binding protein (PCBP2)bind to the type I IRES of picornavirus and hence it leads to internal initiation of translation in type I IREs elements. The heterogenous nuclear ribonucleoprotein A1 (hnRNP A1) is an RNA binding protein which binds to the 5 UTR of HRV2 and regulates transcription (Lin et al, 2009). The picornavirus infection has lowered the level of host cell transcription and hence there is a increase in the number of viral RNA molecules. The nuclear localization signal (NLS) present in the poliovirus 3D protein targets the 3CD precursor to the nucleus for autocatalytic final maturation and allow the release of 3C protease (Bedard and Semler, 2004).) Picornavirus RNA replication: The new positive and negative strand viral RNA are synthesised by using the viral encoded RNA dependent RNA polymerase 3D. The RNA dependent RNA polymerase 3D acts as a protein primer and forms VPg-pU-pU which initiates the viral RNA replication and the process is known as VPg uridylylation. When VPg uridylylation is done, the poly(A) tract at the 3 end acts as an initiation site for the synthesis of negative strand RNA synthesis. The negative strand developed act as a template for the synthesis of new viral positive RNA strand by cap independent translation. Many or numerous copies of positive viral RNA strand can be synthesised from a single negative strand. In picornaviruses, there are numerous RNA sequences and secondary structures within the 5 non coding region which are essential for the RNA replication. The viral protein 3CD and the host protein, PCBP binds at the 5 cloverleaf structure of the non coding region. The cellular host protein PCBP binds to loop b and the 3CD binds to loop d of the 5 cloverleaf structure. A ternary complex is formed when 3CD and PCBP2 binds with the cloverleaf structure along with the viral RNA to form RNA replication. When PCBP2 was depleted, there was poor RNA synthesis in poliovirus and hence it was recognised that PCBP2 play a vital role in RNA replication. PABP is a cellular protein which binds to the poly(A) tract at the 3 end of viral RNA interact with 5 cloverleaf structure of PCBP2 and the viral protein 3CD. When the PABP at the 3 end interact with PCBP2 at the 5 end, the viral RNA may interact and hence there is an initiation of replication process. Even the 3AB and 3CD proteins interact at the 5 cloverleaf structure to each other there by initiating viral RNA replication. The cis-acting replication element (cre) was found within the coding region of picornavirus genomic RNA which is required for viral replication and viability. In aphthovirus the cre structure is at the non coding region of the 5 end. The cre sequence AAACA was found in the conserved hairpin structure at the coding region of picornaviruses required for RNA replication. The cre elements act as a binding site for viral replication proteins and also as a template for VPg uridylylation. At cre element, VPg is more efficient than at the poly(A) tract and uridylylation on cre structure leads to only positive strand RNA synthesis and for uridylylation and replication initiation of the minus strand uses the poly(A) tract. The interaction between 3CD with cre and cloverleaf RNA may arrange the viral RNA structurally for efficient RNA replication. The 3 NCR and the 3 poly(A) tract forms the site of replication for minus strand RNA synthesis. The poly(A) tract at the 3 end functions for virus viability, impart stability and also for the efficient RNA replication by interacting with viral RNA at the 5 end (Bedard and Semler, 2004). The negative strand is first formed by using the VPg protein primer and the VPg plays an important role in replication. The enzymatic activity of 3D polymerase has lead to VPg uridylylation which covalently couples with the tyrosine residue in the VPg protein. The 3 poly (A) tract template may also involve other host proteins for replication. The 2C bind at the 3 end the negative strand showing ATPase activity. The RNA polymerase 3D unwinds to develop viral RNA synthesis (Bedard and Semler, 2004).

Roosevelt and the Great Depression Essay -- History Politics Historica

Roosevelt and the Great Depression The Great Depression of the 1930’s was a great blow to America especially after the seeming prosperity of the twenties. The depression was a result not of false prosperity in the twenties, although the distribution of wealth was very uneven the affluence was very real, but rather from a lack of economic and political maturity to address the problems either before 1929 or as a cure post 1929. The Great Depression is often seen as a result of the twenties when rather it was a failure of the thirties. If the necessary policies had been drawn up in the twenties there would have been widespread hatred for these policies by the wealthy ruling class. This would have made them impossible to implement. It is only during the depression that they became a remote political possibility. Since most of these measures were never tried by either Hoover or Roosevelt we can only speculate as to the level of political acceptance such measures would have encountered. Roosevelt's main measure in combating the Great Depression was the implementation of the New Deal. When Roosevelt excepted the presidential nomination he said â€Å"I pledge you, I pledge myself, to a new deal for the American people†. The New Deal, which was never clearly defined, became the label for the measures undertaken to combat the depression. This New Deal sparked off one of the most concentrated bursts of legislation in American history. In 1933 Roosevelt took up office in the face of an economic crisis. Massive unemployment had swept the country and a banking sector in crisis. â€Å"The New Deal was already in the oven, only half-baked, but it had to be served quickly† it was perhaps for this reason that the barrage of legislation lacked a cohe... ... reversing the policy of fiscal conservatism into one of increased defence spending which brought a boom to many industries. Many of the gains of Roosevelt’s actions are in the long term. Even though many of his government agencies ceased to exist some key reforms were made, especially with regard welfare and the banking sector. The New Deal, though of dubious economic value, set in motion long-range trends toward governmental expansion and modernisation. Works Cited: Burns, J.M. 1956, Roosevelt the Lion and the Fox 1882-1940: Volume One, Harcourt, Brace & World, New York Conkin, P.H. 1968, The New Deal, Routledge American History, London. Graham, O.L. 1967, An Encore for Reform: The Old Progressives and The New Deal, Oxford University Press, New York. Hill, C.P. & Fell, G.H. (eds), 1975, Franklin Roosevelt and the New Deal, Edward Arnold, London

How Much Is the Effect of Acid Rain Environment? Essay

Acid rain is mixed with certain chemicals, including Hydrocarbons. Nitrogen Oxides are created naturally from bacteria, volcanoes and lightening. From the burning of fossil fuels we get chemicals like Sulphur Dioxide, Sulphur Trioxide and Carbon Dioxide. When Carbon Dioxide is mixed with water we get Carbonic Acid. When Nitrogen Oxides are mixed correctly with water it can create Nitric Acid. When Sulphur Dioxide is mixed with water it creates Sulphurous Acid. And when Sulphur Trioxide is mixed with Sulphur Dioxide and water it can create the lethal Sulphuric Acid. Ozone Depletion also impacts on this as its releasing CFC’s which then add to the impact from acid rain. Â  Acid rain can corrode metal structures such as railway tracks and overhead power cables. It can also erode cement, marble and especially limestone buildings. It can take a longer time in some cases due to what the material is, but the evidence is shown a lot of most buildings. Acidic water affects the growth and health of fish and other aquatic life, by reducing the pH of the water and impacting the health and breeding success of the fish and aquatic invertebrates. Death of aquatic organisms due to damaged exoskeleton development as the calcium compounds become more soluble. So there is less calcium in the food chain (for fish bones and birds egg shells). PH5 mucus on fish gills, pH4 species decline. It also dissolves metals out of sediments and rocks to increase the metals (lead) in the water. These can be taken up by the plants and contaminate the associated seeds or fruit. Phytotoxicity is a toxic effect by a compound on plant growth. Such damage may be caused by a wide variety of compounds, including trace metals, pesticides, salinity, phytotoxins or allelopathy as acids damage enzyme function cells in stomata, root hairs. Seed germination is inhibited by acidic conditions. Lichens are especially sensitive to acids. Osmosis, in the water, will affect the aquatic life as well as respiration in the fish themselves, it can harm them. Acid rain impacts crop growth and soil structure. It can affect the growth of plants as many plants have a specific soil pH for optimum growth. In addition it can leach nutrients out of the topsoil, into the groundwater and into the water table. The leaching of nutrients from the soil makes them unavailable to plant life. Acid precipitation can change the ionic balance in clayey soils, affecting their structure. The pollutants that cause acid rain, sulphur dioxide and nitrogen oxides, are inhaled and cause an increase in heart and lung conditions (and premature death). Respiratory illness such as asthma and bronchitis are more common and can lead to pulmonary (heart) disease. Acid rain can also harm organisms by causing other changes that are harmful to living organisms. Soil can be deflocculated by acids where the particles that form peds separate causing the peds to collapse, filling the soil spaces and making the soil less permeable. Lime soils are less affected. Acids increase solubility of ions of metals such as Ca2+ and Al3+ ions which poison organisms. Heavy metals such as lead and mercury are more soluble under acidic conditions, this bio accumulates and bio magnify. (Affect animal’s nervous systems, liver and kidneys) Organisms have an increased susceptibility to pests and disease, waxy cuticles of leaves are removed. Nutrient recycling is reduced as soil bacteria are killed by acid and heavy metal bioaccumulation. Plant diversity is affected, less tolerant species removed first. The Convention on Long-Range Transboundary Air Pollution, often abbreviated as Air Pollution or CLRTAP, is intended to protect the human environment against air pollution and to gradually reduce and prevent air pollution, including long-range transboundary air pollution. The whole point of this organisation is to gradually reduce and prevent air pollution. Parties develop policies and strategies to combat the discharge of air pollutants through exchanges of information, consultation, research and monitoring.

Marketing mix in a business

Paraded Sahara Part 1 (a) The marketing mix is a strategy tool used to formulate a plan for product development and promotions in a business. Examining the mix for the company Bottle Green may help Simon Speers, Company Owner, see the strengths and weaknesses of the business whilst considering its growth potential. The four core elements of marketing mix are product, place, price and promotion.Products: A range of cordial drinks, mostly elderflower but has a growing range including flavors such as, ginger and lemongrass, Cox's apple and Victoria plum. The product is tangible and has a core benefit of quenching thirst. The quality, brand name and packaging may also be a benefit to a consumer who considers their image and how others perceive them. The product life cycle as many others will go through the five phases; development, introduction, growth, maturity and decline. This is why it is important for the company to have new products lined up in order to boost sales.Place: Bottle Gr een distributes to and sells products in supermarkets, Waitress being he single biggest customer, also to tea rooms and small outlets. The company also exports to Sweden and Canada at present. Bottle Greens distribution is out-sourced, this may be a benefit as having another company deal with the logistical area of the business could save time and enable that time to be focused on a more valuable area, such as promotions. Price: Bottle Green Cordial drink is priced Just under E, I assume this is a cost based price.Simon Speers describes the bottle as ‘chic' and hopes for it to be on the dinner tables of the nations middle class. With this premium rand image and quality product it has a competitive price when comparing it to other rival brands. For example, the sparkling soft drink produced by company Seller is currently priced at E. 25 for a mall bottle and Bottle Greens price for a similar product is E. 39 for a mall bottle. Promotion: Bottle Green has a good brand image and has built up brand awareness amongst customers.Not only does the business have a great social media profile, it also sends out samples and recipes to TV chefs who are key influencer in the public eye. Jamie Oliver and Delia Smith being two TV hefts who have given positive feedback and influenced sales to rise. Another area I would like to focus on is purchasing. The bulk of Bottle Greens elderflower comes from the company farm in Clotheshorse and the rest is imported from Hungary. Sugar forms the basis of the cordial drink and this is a problem for Speers.Currently purchasing sugar at Euro-sugar price which is higher than world-sugar price by approximately 2% per tone. At present the business is purchasing at a fixed price but any movement in the wrong direction on the foreign exchange markets will mean further expense for Bottle Green. Part 1 (b) Expert Jeanine Woodcock, managing partner at Bottle Green has some suggestions which mainly focus on promotions of the business. Engaging with customers through the social media profile where customers can discuss stories in reference to the products was one suggestion.This will encourage existing and new customers to advertise the business via word of mouth and the internet. By doing this alongside another of Woodcocks suggestions, researching current customer interests and behavioral patterns, Bottle Green will have a deeper understanding of its statistics and core demographic. This will enable the business to see its strengths and weaknesses in promotions and take action where necessary. The expert also suggests sponsoring a typically British unique and authentic event. This could potentially engage more customers and raise brand awareness which should in turn increase sales.Another expert Joe Whiteouts, deputy director at Bottle Green makes suggestions that focus on product, place and promotion. One suggestion was elderflower capsules that could be added to water and be sold where water based drinks are prepared, for example, offices that use water dispensers. This product development will enable the business to target a new market with a unique new product. Another suggestion was to target the vending machine market, this would put the product in a new place where it would compete with the competition of other soft drink retailers.The final suggestion was to supply schools with recipes in May and June when elderflower is in blossom. The idea that this campaign could be competition based would give potential customers an incentive to engage with the product. It was also suggested that the idea could be pitched as a new healthier alternative to the normal range, this would allow the business to branch out into yet another area of the market. These suggestions could possibly raise brand awareness and bring new customers which would in turn bring more revenue.The business would also have new products in the market beginning a new life cycle. Part 1 (c) The weaknesses I can see in Bottle Greens marketing mix are a lack of product range, the high purchase cost of sugar and the lack of promotion. Firstly I would take the advice of the expert Joe Whiteouts and introduce a new product and a healthier alternative range. This would bring interest from new areas in the market and potentially increase sales. Secondly I would work on the current promotional schemes, I would take the advice of both experts when it comes to promoting the business.Using the social media profile is possibly the quickest, most efficient way to advertise and promote the company in a finance friendly manner. Thirdly sponsoring events and school campaigns are also good ideas, not only British events and British schools but also International events and schools to encourage the potential growth overseas. Fourthly I would branch out and advertise on TV, this is a age opportunity to reach out to millions of potential customers. Finally I would do extensive research on sugar suppliers and try to find a cheape r alternative, this could save the company money and also increase gross profit.I would achieve these goals by working with specific areas in the business such as, the product development team, purchasing team, marketing and promotions team and also the two experts to create new products with a marketing strategy to launch the business into its next level. If all the suggestions were carried out and successful I believe that with rotgut development, increased brand awareness and promotional advertising the business would be in a better financial position and potential growth not only in the I-J but also overseas would look encouraging.